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Identification of Astyanax altiparanae (Teleostei, Characidae) in the Iguaçu River, Brazil, based on mitochondrial DNA and RAPD markers Genet. Mol. Biol.
Prioli,Sônia M.A.P.; Prioli,Alberto J.; Júlio Jr.,Horácio F.; Pavanelli,Carla S.; Oliveira,Alessandra V. de; Carrer,Helaine; Carraro,Dirce M.; Prioli,Laudenir M..
Astyanax fishes are among the most important food-web components of South America rivers. In the Iguaçu River basin, the Astyanax genus is represented mainly by endemic species. For millions of years, that hydrographic basin has been geographically isolated from the Paraná River basin by the Iguaçu Falls. Recently, a species from the Upper Paraná River basin identified as Astyanax bimaculatus was revised and described as a new species named Astyanax altiparanae Garutti & Britski, 2000. Fauna endemism and geographic isolation triggered interest in investigations to evaluate the identification and genetic relatedness among two A. altiparanae populations from the Upper Paraná River basin and the population identified as A. bimaculatus in the Iguaçu...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Astyanax; Fish; D-loop; Control region; Mitochondrial DNA; RAPD.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400011
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The transcription factor Snf1p is involved in a Tup1p-independent manner in the glucose regulation of the major methanol metabolism genes of Hansenula polymorpha Genet. Mol. Biol.
Oliveira,Marcos A.; Genu,Victor; Salmazo,Anita P.T.; Carraro,Dirce M.; Pereira,Gonçalo A.G..
Hansenula polymorpha is a methylotrophic yeast widely employed in biotechnology as a ''protein factory''. Most promoters used for heterologous protein expression, like MOX (methanol oxidase) and DAS (di-hydroxy acetone synthase), are involved in the peroxisomal methanol metabolism (C1 metabolism) and are under strong glucose repression. Interestingly, the MOX promoter is subjected to glucose regulation also in Saccharomyces cerevisiae, a non-methylotrophic yeast in which this phenomenon is well studied. In this species, the transcription factor Tup1p plays an essential role in glucose repression of several genes. This effect is counteracted by the activator Snf1p when glucose is exhausted from medium. Therefore, to test whether this regulatory circuit has...
Tipo: Info:eu-repo/semantics/article Palavras-chave: TUP1; SNF1; Hansenula polymorpha; Glucose repression; MOX.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000400017
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